PrimerEvalPy: a tool for in-silico evaluation of primers for targeting the microbiome

The selection of primer pairs in a sequencing-based investigation of the microbiome can greatly influence the results, manifesting the need for a tool to analyze in silico its performance before the sequencing process. Clinical Relevance— PrimerEvalPy is relevant because its configurability enables the analysis of any target loci and any microbial ecosystem, detecting the best primer pairs to describe the microbiome diversity in any clinical condition.